Facts-enriched characterization of mobile populations. Morphological information from illustrations or photos can add towards the richness of flow cytometry info. Such as, the figure shows an normally common apoptosis assay applying Annexin V and PI, adding mobile imaging to characterize cells in Each and every inhabitants to expose morphologically distinctive features. These insights couldn't have been received from multiplex staining by itself.
Regardless of whether you’re a newbie or a professional, examine our sources to progress your exploration and expertise in flow cytometry.
Merge acoustic concentrating engineering with higher-parameter analysis for adaptable spectral unmixing and conventional workflows.
The award-successful Invitrogen Attune CytPix Flow Cytometer brings together acoustic focusing using a superior-speed brightfield camera for simultaneous significant throughput flow cytometry and large resolution brightfield imaging.
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Listed here, a seasoned user has gated singlets confidently. Soon after analyzing the manual singlet gate, the CytPix image-derived parameter ParticleCount reveals this gate is made up of in excess of 4% aggregates.
Pair your Attune flow cytometer with an autosampler for amplified workflow performance, and include robotic integration for maximum throughput.
Our researchers will style and design panels for you personally as over and over you require—totally free, no product purchase essential.
The Attune NxT cytometer will be the reputable workhorse instrument your lab can trust in for consistent and reliable general performance. The Attune CytPix product incorporates a large-speed brightfield camera and AI-driven image Evaluation program, with modern updates like machine Finding out equipment and an intuitive person interface, enabling Improved information precision, accuracy, and a lot quicker insights into sample biology.
Most likely most importantly, these situations include cells of Evidently different phenotypes which could lead to incorrect conclusions relating to double constructive activities (especially in unusual populations).
AI-driven automated image Evaluation with the Attune Cytometric Software package interprets occasion capabilities into distinct morphology parameters that may be coupled with normal fluorescence and scatter parameters to expose highly reproducible inhabitants improvements and one of a kind subsets.
We Earlier demonstrated the strength of imaging Vehicle-T/Ramos mobile interactions. Enable’s have a look at just the inhabitants of best fascination, the double constructive activities, To find out more. We are able to now use prolonged morphological parameters (below, circularity and skewness of intensity) to even further analyze the functions of these populations and refine gating on these events, raising details robustness.
Leverage the power of Sophisticated technology and innovation to accelerate your discoveries. Believe in from the Attune Flow Cytometer relatives to deliver the dependability, velocity, and precision that set your function aside.
No wash/no lyse leukocyte Examination with imaging. Cells have been acquired from 24-hour-previous blood by dilution in one mM EDTA ( Skilled UX/UI allows consumers to prepare AI versions for expanded apps. (A) The “Train Model” element supplies annotation equipment and actual-time model schooling accuracy.
Gating on these extended parameters permits you to quickly and correctly recognize populations of fascination and ensure gating tactic with little if any handbook review. The Attune software package features access to state-of-the-art device Finding out analysis applications within just an expertly built user interface, which makes it exceptionally simple to operate.
As the mobile line had Earlier been analyzed on the Attune CytPix Flow here Cytometer, the scientists went back to the images and had been capable of doc the microbial an infection not less than 5 times earlier.
Agilent Benefit Guarantee assures you at the very least a decade of instrument use through the day you buy. In any other case, We are going to credit history you With all the residual price of the procedure towards an upgraded model.
Right here, we demonstrate that by gating strategies utilizing graphic-primarily based quantitative parameters can distinguish interacting cells from coincident events to far more precisely analyze interacting cells.